Comparison of DNA damage in granulosa cells of women undergoing controlled ovarian stimulation in in vitro fertilization protocols with the recombinant human follicle-stimulating hormones Corneumon®, Gonal-F®, Pergoveris® and Puregon®: a randomized trial.

PURPOSE: To compare the DNA damage in granulosa cells (GCs) of women undergoing ovarian-stimulated cycles with four widely used recombinant human follicle-stimulating hormones (rhFSH) in in vitro fertilization (IVF) protocols (Corneumon®, Gonal-F®, Pergoveris® and Puregon®). METHODS: A randomized trial was carried out at a Mexican hospital. GCs were isolated from 18 women with infertility undergoing assisted reproductive techniques (ART). Four controlled ovarian stimulation (COS) protocols including Corneumon®, Gonal-F®, Pergoveris® or Puregon® were used. GCs DNA damage was assessed by the Comet assay. Two parameters were measured: comet tail length (CTL), and Olive tail moment (OTM, the percentage of DNA in the tail multiplied by the distance between the center of the tail and head). RESULTS: Use of the different hrFSH in COS caused variable and statistically significant levels of DNA damage in GCs of infertile women. CTL was similar in the Corneumon® and Pergoveris® groups (mean values of 48.73 and 55.18, respectively) and Corneumon® CTL was significantly lower compared to the Gonal-F® and Puregon® groups (mean values of 61.98 and 91.17, respectively). Mean OTM values were significantly lower in Corneumon® and Pergoveris® groups, compared to Gonal-F® and Puregon® groups (25.59, 27.35, 34.76, and 47.27, respectively). CONCLUSION: Use of Corneumon® and Pergoveris® in COS caused statistically significantly lower levels of DNA damage in GCs of infertile women undergoing ART, which could potentially correlate with better reproductive outcomes.

Evaluación de los parámetros seminales en varones atendidos en centros de fertilidad de Perú y México / Evaluation of semen parameters in men attending at fertility centers in Perú and México

OBJETIVO: Determinar el parámetro espermático más frecuente en los pacientes atendidos en la ciudad de Chiclayo (27 msnm) y México D.F (2240 msnm). Caracterizar y comparar los parámetros espermáticos de los pacientes atendidos en las dos ciudades. MATERIAL Y MÉTODOS: Estudio prospectivo realizado en el Laboratorio de Andrología de IN VITRO GESTAR, Chiclayo - Perú (n=102 pacientes, Normozoospermicos =56) y HISPAREP, Centro de Reproducción Asistida, Hospital Español, México D.F (n=115 pacientes, Normozoospermicos=13), durante los meses de Octubre 2013 a Agosto 2014. Las muestras seminales fueron analizadas siguiendo los criterios de la OMS 2010. Para la determinación de la mediana (test no paramétrico) se utilizo el software SPSS 22. RESULTADOS: Se determino que la morfología espermática (78,3 %) y la movilidad progresiva (24,5 %) fueron los parámetros seminales afectado más frecuentes en México y Perú respectivamente. Se encontraron diferencias significativas (P=0,000) en la mediana en los parámetros de concentración (106/ml) (36,60 vs 76,50), movilidad progresiva (%) (33,00 vs 49,20), morfología normal (%) (2,00 vs 5,00), vitalidad (%) (75,00 vs 85,00) en la población total de México y Perú respectivamente. Se encontraron diferencias significativas en la mediana de la movilidad progresiva (%) (44,00 vs 55,90 P=0,003) de la muestras normozoospermicas en la población de México y Perú respectivamente. CONCLUSIONES: La causa principal de infertilidad masculina difiere de una población a otra. Se demostró que existen diferencias significativas en los parámetros seminales de los varones atendidos en Chiclayo y México D.F. Entre los pacientes normozoospermicos se encontró diferencias significativas en el parámetro de la movilidad progresiva. Podemos atribuir estas diferencias debido a las variaciones geográficas, diferencia de altitudes, endocrinas, genética, étnica, tipo de vida que existen entre ambos países

OBJETIVE: To determine the seminal parameter most frequent in patients attending in the city of Chiclayo (27 m) and Mé- xico D.F (2240 m). Characterize and compare the sperm parameters of the patients attending in the two cities. MATERIAL AND METHODS: Prospective study conducted at the Laboratory of Andrology "In Vitro Gestar" (Chiclayo - Perú) (n=102 patients, Normozoospermic=56) and HISPAREP, Center for Assisted Reproduction, Spanish Hospital, Mexico D.F (n=115 patients, Normozoospermic = 13), from October 2013 to August 2014. The semen samples were analyzed according to the criteria of WHO 2010. For determination of the median was used SPSS 22 software. RESULTS: It was determined the sperm morphology (78.3%) and progressive motility (24.5%) were the semen parameters affected most frequent in México and Perú respectively. We determined significant differences (P=0.000) in the median in the parameters of concentration (106 /ml) (36.60 vs 76,50), progressive motility (%) (33.00 vs 49.20), normal morphology (%) (2.00 vs 5.00), vitality (%) (75.00 vs 85.00) in the total population of México and Perú, respectively. We determined significant differences in the median of the progressive motility (%) (44.00 vs 55.90 P=0.003) of the normozoospermic samples in the population of México and Perú, respectively. CONCLUSIONS: The main cause of male infertility differs from one population to another. It was shown that there significant difference in the semen parameters of the male attending in Chiclayo and México D.F. Among the patients was found significant differences in the progressive motility. We can attribute these differences due to geographical variations, altitude differences, endocrine, genetic, ethnic, lifestyle between the two countries